B-hHAVCR2 (TIM3) Mice

Basic Information

Strain Name
C57BL/6-Havcr2tm1(HAVCR2)Bcgen/Bcgen
Stock Number
110018
Common Name
B-hHAVCR2 (TIM3) Mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
Havcr2 (hepatitis A virus cellular receptor 2)
Species
C57BL/6
Appearance
Black
Genotypes
Homozygous

Description

T-cell immunoglobulin domain and mucin domain-3 (TIM3) is an activation-induced inhibitory molecule involved in immune tolerance and T-cell exhaustion in chronic viral infection and cancers. TIM3 maturation and cell surface expression is facilitated by forming a heterodimeric interaction with CD66a. Co- blockade of CD66a and TIM3 enhanced anti-tumor immune responses and eliminated tumors in mouse colorectal cancer models.

Targeting Strategy

Details

Phenotype

Protein Expression Analysis

Cells in ascites from both wild type (WT)C57BL/6 and homozygous B-hTIM3 mice were analyzed by flow cytometry. Mouse TIM3 + cells were detectable in non-T and non-B cells from the WT mice, while human TIM3 + cells were detectable in non-T and non-B cells from the homozygous B-hTIM3 mice.

Splenocytes from both wild type (WT)C57BL/6 and homozygous B-hTIM3 mice were analyzed by flow cytometry. Mouse TIM3 + T cells were detectable in the WT mice, while human TIM3 + T cells were detectable in the homozygous B-hTIM3 mice.

 

Application

Human TIM3 mAb Efficacy Evaluation (MC38 cell line)

B-hHAVCR2TIM3-Mice-human-tim3-mab-efficacy-evaluation

Murine colon cancer MC38 cells were subcutaneously implanted into homozygous B-hTIM3 mice. Mice were divided into control and treatment groups (n = 5) when the tumor volume was about 150±50 mm3. The anti-human TIM3 antibody significantly inhibited tumor growth in homozygous B-hTIM3 mice, suggesting that the B-hTIM3 mouse model is an effective tool for in vivo hTIM3 antibody efficacy studies. (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.

References

1. Nat Immunol. 2003 Nov;4(11):1102-10. Epub 2003 Oct 12.
2. Nat Immunol. 2005 Dec;6(12):1245-52. Epub 2005 Nov 13.
3. Immunity. 2007 Mar;26(3):311-21.
4. J Immunol. 2010 Feb 15;184(4):1918-30. doi: 10.4049/jimmunol.0903059. Epub 2010
Jan 18.

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