Basic Information

Strain Name
C57BL/6-IL17atm1(hIL17A)/Bcgen
Stock Number
B-EM-038
Common Name
B-hIL17A mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
IL17A (interleukin 17A)
Species
C57BL/6
Appearance
Black
Genotypes
Homozygous

Description

IL17A encodes the pro-inflammatory cytokine IL-17A that is a member of the interleukin-17 family. IL-17A is produced by activated T helper (Th17) cells and certain cell types of innate immune system (Cua and Tato, Nat Rev Immunol. 2010, 10(7):479-89). IL-17A functions as either a homodimer or a heterodimer with other IL-17 family members, e.g. IL-17F and signals through the IL-17 receptor (IL-17R), which is composed of IL-17RA and IL-17RC, to induce chemokine and cytokine production. High level of IL-17A is associated with several autoimmune diseases, including psoriasis, multiple sclerosis, and rheumatoid arthritis. IL-17A also plays a central role in host defense against bacterial

Gene Targeting

B-hIL17A-Targeting

Details

Phenotype

Protein Expression Analysis

B-hiL-17A-Splenocytes

Splenocytes were isolated from C57BL/6 and B-hIL-17A mice (n=3) and profiled by flow cytometry. As shown in the panels above, leukocytes subpopulations in homozygous B-hIL-17Amice are similar to those in the C57BL/6 mice, indicating that development of T, NK, Monocyte, DC and macrophage cells are not affected by the knock-in of hIL-17A.

Analysis of T Cell Subpopulation in B-hIL-17A Mice

Analysis-T-Cell-Subpopulation-B-hIL-17A

Lymphocytes were isolated from spleen in C57BL/6 and B-hIL-17A mice (n=3). T cells subpopulations were profiled by flow cytometry. As shown above, the T cell subpopulations in homozygous B-hIL-17A mice are similar to those in the C57BL/6 mice, indicating that T cell development is not affected by the introduction of hIL-17A.

Analysis of Leukocytes Cell Subpopulation in B-hIL-17A Mice

B-hiL-17A-Blood-Isolated

Blood were isolated from C57BL/6 and B-hIL-17A mice (n=3) and profiled by flow cytometry. As shown in the panels above, leukocytes subpopulations in homozygous B-hIL-17Amice are similar to those in the C57BL/6 mice, indicating that development of T, NK, Monocyte, DC and macrophage cells are not affected by the knock-in of hIL-17A.

Analysis of T cell Subpopulation in B-hIL-17A Mice

Lymphocytes-B-hIL-17A

Lymphocytes were isolated from blood in C57BL/6 and B-hIL-17A mice (n=3). T cells subpopulations were profiled by flow cytometry. As shown above, the T cell subpopulations in homozygous B-hIL-17A mice are similar to those in the C57BL/6 mice, indicating that T cell development is not affected by the introduction of hIL-17A.

Blood Routine Test

B-hiL-17A-Blood-Routine-Test

Complete blood counts (CBC)

Blood from the C57BL/6 and B-hIL-17Amice (n=3) was collected and analyzed. As shown above, there  are no significant differences in terms of CBC between C57BL/6 and B-hIL-17A mice.

Blood Biochemical test

B-hiL-17A-Serum

Serum ALT/AST levels of C57BL/6 and B-hIL-17Amice were tested (n=3). Results indicate that there are no significant differences in ALT/AST levels between C57BL/6 and B-hIL-17A mice.

Application of EAE Model

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