B-hPD-1/h4-1BB Mice

Basic Information

Strain Name
C57BL/6-Pdcd1 tm1(PDCD1) Cd137 tm1(CD137) /Bcgen
Stock Number
120516
Common Name
B-hPD-1/h4-1BB mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
PD-1 (Programmed death-1); TNFRSF9 (Tumor necrosis factor receptor superfamily, member 9)
Species
C57BL/6
Appearance
Black
Genotypes
Homozygous
Gene Description

Targeting Strategy

 

Details

Phenotype

Protein Expression Analysis

Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hPD-1/h4-1BB mice were analyzed by flow cytometry. Mouse 4-1BB+ T cells were detectable in the WT C57BL/6 mice, while human 4-1BB+ T cells were detectable in the homozygous B-hPD-1/h4-1BB mice.

Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hPD-1/h4-1BB mice were analyzed by flow cytometry. Mouse PD-1+ T cells were detectable in the WT C57BL/6 mice, while human PD-1+ T cells were detectable in the homozygous B-hPD-1/h4-1BB mice.

Application

Combination therapy of PD-1 (Keytruda) mAb and 4-1BB (Urelunab Analog ) mAb

Murine colon cancer MC38 cells were subcutaneously implanted into homozygous B-hPD-1/h4-1BB mice. Mice were grouped (n=5) when the tumor size was approximately 150 ± 50mm3 (n=6). Anti-hPD-1 antibody and anti-h4-1BB antibody significantly inhibited tumor growth in the homozygous B-hPD-1/h4-1BB mice, suggesting that B-hPD-1/h4-1BB mouse model is an effective tool for in vivo efficacy evaluation of hPD-1 and h4-1BB antibody combination therapy. (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM

References

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  3. Nat Commun. 2017 Feb 6; 8: 14369. doi: 10.1038/ncomms14369.
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  5. J Exp Med. 2000 Oct 2;192(7): 1027-34.
  6. Science. 2001 Jan 12;291(5502): 319-22.
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