mRNA Expression Analysis
RT-PCR analysis of the B-hPD-1/hTIM3 humanized mice. The splenocytes of the B-hPD-1/hTIM3 homozygous mice showed expression of human PD-1 and TIM3 mRNA but not mouse PD-1 and TIM3 mRNA.
Protein Expression Analysis
Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hPD-1/hTIM3 mice were analyzed by flow cytometry. Mouse TIM3+ and PD-1+ T cells were detectable in the WT C57BL/6 mice, while human TIM3+ and PD-1+ T cells were detectable in the homozygous B-hPD-1/hTIM3 mice.
Combination Therapy of PD-1 mAb (Keytruda) and TIM3 mAb
Murine colon cancer MC38 cells were subcutaneously implanted into homozygous B-hPD-1/hTIM3 mice. Mice were grouped when the tumor size was approximately 150±50 mm3 (n=6). Anti-TIM3 antibody and the anti-hPD-1 antibody Keytruda shows obviously inhibitory effects respectively. Combination of anti-hTIM3 antibody and the anti-hPD-1 antibody Keytruda shows better inhibitory effects than single treatment, suggesting that B-hPD-1/hTIM3 mouse is a powerful tool for in vivo evaluating combination therapy efficacy of hPD-1 antibody and hTIM3 antibody.
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