B-hPD-L1/hCD27 Mice

Basic Information

Strain Name
C57BL/6-Cd274tm1(CD274) Cd27tm1(CD27)/Bcgen
Stock Number
120537
Common Name
B-hPD-L1/hCD27mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
CD27(CD27 molecule); Cd274 (CD274 antigen)
Species
C57BL/6
Appearance
Black
Genotypes
Homozygous

Targeting Strategy

Details

mRNA Expression Analysis

RT-PCR analysis of the B-hPD-1/hCD27 humanized mice.

The splenocytes of the B-hPD-1/hCD27 homozygous mice showed expression of human PD-1 and CD27 mRNA but not mouse PD-1 and CD27 mRNA.

Protein Expression Analysis

Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hPD-1/hCD27 mice were analyzed by flow cytometry.

Mouse CD27+ T cells were detectable in both WT C57BL/6 and the homozygous B-hPD-1/hCD27 mice, while human CD27+ T cells were detectable in the homozygous B-hPD-1/hCD27 mice. This might result from the cross-recognition of hCD27 by anti-mCD27 antibodies.

Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hPD-1/hCD27 mice were analyzed by flow cytometry.

Mouse PD-1+ T cells were detectable in the WT C57BL/6 mice, while human PD-1+ T cells were detectable in the homozygous B-hPD-1/hCD27 mice.

References

  1. Clin Cancer Res 2016; published online Nov 8. DOI:10.1158/1078-0432.CCR-16-1741
  2. Blood First Edition paper, May 11, 2010; DOI 10.1182/blood-2010-02-271874.
  3. Journal of Clinical Oncology 35, no. 18 (June 20 2017) 2028-2036. DOI: 10.1200/JCO.2016.70.1508
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