B-hTNFRSF18 (GITR) Mice

Basic Information

Strain Name
C57BL/6-Tnfrsf18tm1(TNFRSF18)Bcgen/Bcgen
Stock Number
110012
Common Name
B-hTNFRSF18(GITR) mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
Tnfrsf18 (tumor necrosis factor receptor superfamily, member 18)
Species
C57BL/6
Appearance
Black
Genotypes
Homozygous

Description

TNFRSF18 (TNF Receptor Superfamily Member 18) is also known as Glucocorticoid-induced TNFR-related protein (GITR), which is expressed on many immune cells including T cells. As a co-stimulatory signal of T cells, TNFRSF18 is upregulated upon the activation of T cells, and in turn promotes T cell proliferation. CD25+/CD4+ regulatory T cell is known to mediate immune tolerance, and GITR agonist antibodies can reverse this immune tolerance, and show anti-tumor effect in multiple tumor models.

Targeting Strategy

Details

Phenotype

Protein Expression Analysis

Splenocytes from both wild type (WT) C57BL/6 and heterozygous B-hGITR mice were analyzed. Mouse GITR+ T cells were detectablby flow cytometrye in both WT C57BL/6 and heterozygous B-hGITR mice, while human GITR+ T cells were only detectable in the heterozygous B-hGITR mice.

Application

Human GITR mAb efficacy evaluation (MC38 cell line)

B-hpd-1-Mice-application

Murine colon cancer MC38 cells were subcutaneously implanted into heterozygous B-hGITR mice. Mice were grouped when the tumor size was approximately 150±50 mm3 (n=6). Three human GITR antibodies differentlially inhibited tumor growth, confirming that the B-hGITR mouse model is a powerful tool for in vivo GITR antibody pharmacological efficacy studies. (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.

References

  1. ONCOIMMUNOLOGY 2017, VOL. 6, NO. 3, e1280645 (14 pages) doi: 10.1080/2162402X.2017.1280645
  2. Cancer Immunol Res; 1(5) November 2013 doi:10.1158/2326-6066.CIR-13-0086
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