B-Il17a-Gluc Mice

Basic Information

Strain Name
C57BL/6-Il17a tm1(Gluc) /Bcgen
Stock Number
B-EM-019
Common Name
B-Il17a-Gluc Mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
Il17; Ctla8; IL-17; Ctla-8; IL-17A
Species
C57BL/6
Appearance
Black
Genotypes
Il17a Gluc (mut/mut)
Development
The Il17a Gluc mice were developed by Beijing Biocytogen Co., Ltd. An IRES-Gluc-SV40-polyA sequence cassette was inserted after the stop codon of the Il17a gene using C57BL/6-derived embryonic stem (ES) cells. This strain was maintained on a C57BL/6 genetic background.

Description

The IL17A is a pro-inflammatory cytokine that, although expressed by several cell types, defines the T helper 17 (TH17) cell subset. IL17A activates signal transduction pathways that recruit neutrophils, promote the production of antimicrobial factors, and drive the inflammatory pathology associated with autoimmune disorders.

Targeting Strategy

B-IL17a-Gluc-mice-Targeting-Strategy

Details

Genotyping Information

Primer Sequence (5’-3’) Tm
(℃)
Product size
(bp)
Il17a Gluc primer 1 AAGCTGGACCACCACATGA 62 WT: 264
Il17a Gluc primer 2 TGAATCCACATTCCTTGCTG 62
Il17a Gluc primer 1 AAGCTGGACCACCACATGA 62 Mut: 390
Il17a Gluc primer 3 GACATTCAACAGACCTTGCATTC 62

Polymerase: Taq

 

 

 

References

1. Colpitts SL; Puddington L; Lefrancois L. 2015. IL-15 receptor alpha signaling constrains the development of IL-17 producing gammadelta T cells. Proc Natl Acad Sci U S A 112(31):9692- 7. [PubMed: 26195801]
2.Taylor PR; Roy S; Leal SM Jr; Sun Y; Howell SJ; Cobb BA; Li X; Pearlman E. 2014.
Activation of neutrophils by autocrine IL-17A-IL-17RC interactions during fungal infection is regulated by IL-6, IL-23, RORgammat and dectin-2. Nat Immunol 15(2):143-51. [PMID:
24362892]
3. Goto Y; Panea C; Nakato G; Cebula A; Lee C; Diez MG; Laufer TM; Ignatowicz L; Ivanov
II. 2014. Segmented filamentous bacteria antigens presented by intestinal dendritic cells drive mucosal Th17 cell differentiation. Immunity 40(4):594-607. [PMID: 24684957]
4. Duhen R; Glatigny S; Arbelaez CA; Blair TC; Oukka M; Bettelli E. 2013. Cutting Edge: The Pathogenicity of IFN-gamma-Producing Th17 Cells Is Independent of T-bet. J Immunol 190(9):4478-82. [PMID: 23543757]
5. Lee Y; Awasthi A; Yosef N; Quintana FJ; Xiao S; Peters A; Wu C; Kleinewietfeld M; Kunder S; Hafler DA; Sobel RA; Regev A; Kuchroo VK. 2012. Induction and molecular signature of pathogenic T(H)17 cells. Nat Immunol 13(10):991-9. [PMID: 22961052]

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