Basic Information

Strain Name
SD-Ldlrtm1/Bcgen
Stock Number
B-TR-004
Common Name
B-Ldlr-KO Rats
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
Ldlr (low density lipoprotein receptor)
Species
Sprague Dawley
Appearance
White
Genotypes
LdlrKO (mut/mut)
Development
The LdlrKO rat was developed using targeted TALEN technology in the Sprague-Dawley strain, and this model possesses a 2 bp deletion within Exon 1 on chromosome 8. This strain was maintained by mating homozygous males with homozygous females on a Sprague-Dawley genetic background.

Description

The Low Density Lipoprotein (LDL) Receptor is a cell-surface receptor presented in clathrin-coated pits that mediates the endocytosis of LDL, the major cholesterol-carrying lipoprotein of plasma. Loss of Ldlr function leads to accumulation of excess LDL in the blood, leading to elevated serum cholesterol, especially when fed a high fat diet, which makes Ldlr knockout rat a good model for studying atherosclerosis.

Targeting Strategy

B-Ldlr-KO-rats-targeting-strategy

Details

Genotyping Information

Primer Sequence (5’-3’) Tm

()

Product size (bp)
LdlrKO primer 1 GTAGTCAGTCACCAGTTGACCCAGT 62 Mut: 875

WT: 877

LdlrKO primer 2 CAGAGGCACAACTATGAAGCTGACC 62

Polymerase: Taq

The fragments need to be verified by sequencing.

References

  1. Afek A; Keren G; Harats D; George J. 2001. Whole body hyperthermia accelerates atherogenesis in low-density lipoprotein receptor deficient mice. Exp Mol Pathol 71(1):63-72. [PubMed: 11502098]
  2. Adachi H; Kondo T; Koh GY; Nagy A; Oike Y; Araki E. 2011. Angptl4 deficiency decreases serum triglyceride levels in low-density lipoprotein receptor knockout mice and streptozotocin-induced diabetic mice. Biochem Biophys Res Commun 409(2):177-80. [PubMed: 21549101]
  3. Babaev VR; Ishiguro H; Ding L; Yancey PG; Dove DE; Kovacs WJ; Semenkovich CF; Fazio S; Linton MF. 2007. Macrophage expression of peroxisome proliferator-activated receptor-alpha reduces atherosclerosis in low-density lipoprotein receptor-deficient mice. Circulation 116(12):1404-12. [PubMed: 17724261]
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