Basic Information

Common Name
B-hB7-H3 MC38
Aliases
CD276, 4Ig-B7-H3, B7H3, B7RP-2
Catalog Number
310704
Cell Type/ Disease
Colon Carcinoma
Species/ Strain
Mus musculus, C57BL/6
Notice
Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines. Our humanized tumor cell lines are for pharmacology services ONLY.

Targeting strategy

The exogenous promoter and human B7-H3 coding sequence were inserted to replace part of murine exon 3 and all of exon 4. The insertion disrupts the endogenous murine B7h3 gene, resulting in a non-functional transcript.

Protein expression analysis

B7-H3 expression analysis in B-hB7-H3 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hB7-H3 MC38 cultures were stained with species-specific anti-B7-H3 antibody. Human B7-H3 was detected on the surface of B-hB7-H3 MC38 cells but not wild-type MC38 cells. The 2-F02 clone of B-hB7-H3 MC38 cells was used for in vivo experiments.

Tumor growth curve

Subcutaneous homograft tumor growth of B-hB7-H3 MC38 cells. B-hB7-H3 MC38 cells (5×105) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6N mice (female, 5-8-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Bodyweight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hB7-H3 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

Recommendations for inoculation in other mouse strains:

1. Cell inoculation amount is recommended to be tried between 5×105-1×107;

2.Inoculated cells can be tried to be suspended with DMEM stock solution.

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