Basic Information

Strain Name
MC38 Vtcn1tm1(hVTCN1)/Bcgen
Stock Number
Common Name
B-hB7-H4 MC38
Colon Carcinoma
Mus musculus, mouse
Culture Properties
Culture medium
DMEM+10% FBS+1% L-Glutamine+1% NEAA+1% Sodium pyruvate+1%HEPES
90% FBS+10% DMSO
Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines.Our humanized tumor cell lines are for pharmacology services ONLY.

Targeting Strategy

Gene targeting strategy for B-CAG-hB7-H4 MC38. The human full-length B7-H4 CDS region was inserted in the B7-h4 exon3~4 of wild-type MC38.


Protein expression analysis

The B7-H4 expression analysis in B-CAG-hB7-H4 MC38 cells by flow cytometry. Single cells were collected from wild-type MC38 and B-CAG-hB7-H4 MC38 cells, and analyzed by flow cytometry with species-specific anti-B7-H4 antibody. Human CD47 was detectable in B-CAG-hB7-H4 MC38 cells but not wild-type MC38 cells.

Tumor Growth Curve & Body Weight Changes

Subcutaneous xenograft tumor growth of B-CAG-hB7-H4 MC38 cells. B-CAG-hB7-H4 MC38 cells and wild-type MC38 cells (5×105) were subcutaneously implanted into the C57BL/6N mice (female, 7-9 week-old, n=5).Tumor size and mice body weight were measured twice a week. (A) Tumor average volume ± SEM, (B) Mice body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5a X b2, where a and b were the long and short diameters of the tumor, respectively. As shown in panel A, B-CAG-hB7-H4 MC38 were able to establish tumor in vivo and can be used for efficacy study.

Tumor expression detection sample information

Protein expression analysis in tumor cells


FMO Control 1: MC38 tumor cells only stained anti-hB7-H4 antibody;

FMO Control 2: B-CAG-hB7-H4 MC38 cells only stained anti-mB7-H4 antibody;

FMO Control 3: MC38 cells only stained anti-hB7-H4 antibody.

B-CAG-hB7-H4 MC38 cells were transplanted into C57BL/6N mice(n=5), and on 42 days post inoculation, certain volumes of tumors as was taken to detect the expression level of the cells. As Shown,human B7-H4 was highly expressed on the surface of tumor cells. B-CAG-hB7-H4 MC38 can be used for in vivo efficacy validation.

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