B-CAG-hEPCAM MC38

Basic Information

Strain Name
MC38 Epcamtm1(hEPCAM)/Bcgen
Stock Number
310708
Common Name
B-CAG-hEPCAM MC38
Disease
Colon Carcinoma
Species
Mus musculus, mouse
Culture Properties
Adherent
Culture medium
DMEM+10% FBS+1% L-Glutamine+1% NEAA+1% Sodium pyruvate+1%HEPES
Frozen
90% FBS+10% DMSO
Notice
Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines. Our humanized tumor cell lines are for pharmacology services ONLY.

Targeting Strategy

Gene targeting strategy for B-CAG-hEGFR MC38.The exons 4-7 of mouse Egfr gene that encode the extracellular domain were replaced by human EGFR CDS region in B-CAG-hEGFR MC38.

Details

Protein expression analysis

The EPCAM expression analysis in B-CAG-hEPCAM MC38 cells by flow cytometry. Single cells were collected from wild-type MC38 and B-CAG-hEPCAM MC38 cells, and analyzed by flow cytometry with species-specific anti-EPCAM antibody. Human EPCAM was detectable in B-CAG-hEPCAM MC38 cells but not wild-type MC38 cells.

Tumor Growth Curve & Body Weight Changes

Subcutaneous xenograft tumor growth of B-CAG-hEPCAM MC38 cells. B-CAG-hEPCAM MC38 cells and wild-type MC38 cells (5×105) were subcutaneously implanted into the C57BL/6 mice (female, 5-8 week-old, n=5).Tumor size and mice body weight were measured twice a week. (A) Tumor average volume ± SEM, (B) Mice body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5a X b2, where a and b were the long and short diameters of the tumor, respectively. As shown in panel A, B-CAG-hEPCAM MC38 were able to establish tumor in vivo and can be used for efficacy study.

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