Basic Information
Strain Name
MC38 Cd274 tm2(CAG-CD274,-luc)/Bcgen
Stock Number
310699
Common Name
B-hPD-L1 MC38(v2)
Disease
Colon Carcinoma
Species
Mus musculus, mouse
Culture Properties
Adherent
Culture medium
DMEM+10%FBS
Frozen
90% Complete medium+10% DMSO
Notice
Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines. Our humanized tumor cell lines are for pharmacology services ONLY.
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Targeting Strategy
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Details
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Phenotyping Information
Phenotypic analysis of B-CAG-hPD-L1 MC38 cell line(v2)
Flow cytometry analysis of human PD-L1 Protein. The hPD-L1, but not mPD-L1, protein was detected in B-CAG-hPD-L1 MC38 cell line (v2).
Tumor Growth Curve
C57BL/6 mice were subcutaneously injected with tumor cells in the right front flank for tumor development. Tumor size was measured two times weekly in two dimensions using a caliper, and the volume was expressed in mm3 using the formula: V=0.5a X b2, where a and b were the long and short diameters of the tumor, respectively.
