Basic Information
Strain Name
MC38 Pdcd1lg2 tm1(CAG-PDCD1LG2)Bcgen
Stock Number
310703
Common Name
B-hPD-L2 MC38
Disease
Colon Carcinoma
Species
Mus musculus, mouse
Culture Properties
Adherent
Culture medium
DMEM+10%FBS
Frozen
90% Complete medium+10% DMSO
Notice
Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines. Our humanized tumor cell lines are for pharmacology services ONLY.
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Targeting Strategy
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Details
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Phenotyping Information
Phenotypic analysis of B-CAG-hPD-L2 MC38 Cell Line
B-CAG-hPD-L2 MC38 Cell Line were analyzed by flow cytometry. The hPD-L2, but not mPD-L2, protein was detected in B-CAG-hPD-L2 MC38 Cell Line.
Tumor Growth Curve
C57BL/6 mice were subcutaneously injected with tumor cells (5E5) in the right front flank for tumor development. Tumor size was measured two times weekly in two dimensions using a caliper, and the volume was expressed in mm3 using the formula: V=0.5a X b2, where a and b were the long and short diameters of the tumor, respectively.
Tumor Cells Analysis
B-CAG-hPD-L2 MC38 and MC38 tumors were analyzed by flow cytometry. Results shows that compared with MC38, the expression of hPD-L2 on the surface of B-CAG-hPD-L2 MC38 tumor cells was significantly increased.
