Basic Information

Common Name
B-hPD-L2 MC38
Aliases
PDCD1LG2, CD273,  PDCD1L2
Catalog Number
310703
Cell Type/ Disease
Colon Carcinoma
Species/ Strain
Mus musculus, C57BL/6
Notice
Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines. Our humanized tumor cell lines are for pharmacology services ONLY.

Description

The mouse PD-L2 gene was replaced by human PD-L2 coding sequence in B-hPD-L2 MC38 cells. Human PD-L2 is highly expressed on the surface of B-hPD-L2 MC38 cells.

Targeting strategy

The exogenous promoter and human PD-L2 coding sequence was inserted to replace part of murine exon 3. The insertion disrupts the endogenous murine PD-L2 gene, resulting in a non-functional transcript.

Protein expression analysis

PD-L2 expression analysis in B-hPD-L2 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hPD-L2 MC38 cultures were stained with species-specific anti-PD-L2 antibody. Mouse PD-L2 was not detected on the surface of wild-type MC38 cells. Human PD-L2 was detected on the surface of B-hPD-L2 MC38 cells but not wild-type MC38 cells. The 1-C11 clone of B-hPD-L2 MC38 cells was used for in vivo experiments.

Tumor growth curve

Subcutaneous homograft tumor growth of B-hPD-L2 MC38 cells. B-hPD-L2 MC38 cells (5×105) were subcutaneously implanted into C57BL/6 mice (female, 5-8-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hPD-L2 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

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