B-hCCR4 MC38

Basic Information

Common Name
B-hCCR4 MC38
Aliases
CC-CKR-4, CD194, CKR4, CMKBR4
Catalog Number
310894
Cell Type/ Disease
Colon Carcinoma
Species/ Strain
Mus musculus, C57BL/6
Notice
Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines. Our humanized tumor cell lines are for pharmacology services ONLY.

Description

The mouse Ccr4 gene was replaced by human CCR4 coding sequence in B-hCCR4 MC38 cells. Human CCR4 is highly expressed on the surface of B-hCCR4 MC38 cells.

Targeting strategy

The exogenous promoter and human CCR4 coding sequence was inserted to replace part of murine exon 2. The insertion disrupts the endogenous murine Ccr4 gene, resulting in a non-functional transcript.

Protein expression analysis

CCR4 expression analysis in B-hCCR4 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hCCR4 MC38 cultures were stained with species-specific anti-CCR4 antibody. Human CCR4 was detected on the surface of B-hCCR4 MC38 cells but not wild-type MC38 cells. The 2-F05 clone of B-hCCR4 MC38 cells was used for in vivo experiments.

Tumor growth curve

Subcutaneous homograft tumor growth of B-hCCR4 MC38 cells. B-hCCR4 MC38 cells (5×105) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6N mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hCCR4 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM).

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