Basic Information

Common Name
B-hCD38 MC38
Aliases
ADPRC1
Catalog Number
310730
Cell Type/ Disease
Colon Carcinoma
Species/ Strain
Mus musculus, C57BL/6
Notice
Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines. Our humanized tumor cell lines are for pharmacology services ONLY.

Description

The mouse Cd38 gene was replaced by chimeric CD38 coding sequence in B-hCD38 MC38 cells. Human CD38 is highly expressed on the surface of B-hCD38 MC38 cells.

Application

B-hCD38 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Target strategy

The exogenous promoter and chimeric CD38 coding sequence was inserted to replace part of murine exon 2 and all of exon 3. The insertion disrupts the endogenous murine Cd38 gene, resulting in a non-functional transcript.

Protein expression analysis

CD38 expression analysis in B-hCD38 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hCD38 MC38 cultures were stained with species-specific anti-CD38 antibody. Mouse CD38 was detected on the surface of wild-type MC38 cells. Human CD38 was detected on the surface of B-hCD38 MC38 cells but not wild-type MC38 cells. The 4-F06 clone of B-hCD38 MC38 cells was used for in vivo experiments.

Tumor growth curve

Subcutaneous homograft tumor growth of B-hCD38 MC38 cells. B-hCD38 MC38 cells (5×105) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6N mice (female, 7-8-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hCD38 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

Recommendations for inoculation in other mouse strains:

1.Cell inoculation amount is recommended to be tried between 5×105-1×107;

2.Inoculated cells can be tried to be suspended with DMEM stock solution.