Basic Information
Description
The mouse Cd38 gene was replaced by chimeric CD38 coding sequence in B-hCD38 MC38 cells. Human CD38 is highly expressed on the surface of B-hCD38 MC38 cells.
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Application
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B-hCD38 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.
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Target strategy
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The exogenous promoter and chimeric CD38 coding sequence was inserted to replace part of murine exon 2 and all of exon 3. The insertion disrupts the endogenous murine Cd38 gene, resulting in a non-functional transcript.
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Protein expression analysis
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CD38 expression analysis in B-hCD38 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hCD38 MC38 cultures were stained with species-specific anti-CD38 antibody. Mouse CD38 was detected on the surface of wild-type MC38 cells. Human CD38 was detected on the surface of B-hCD38 MC38 cells but not wild-type MC38 cells. The 4-F06 clone of B-hCD38 MC38 cells was used for in vivo experiments.
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Tumor growth curve
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Subcutaneous homograft tumor growth of B-hCD38 MC38 cells. B-hCD38 MC38 cells (5×105) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6N mice (female, 7-8-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hCD38 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
Recommendations for inoculation in other mouse strains:
1.Cell inoculation amount is recommended to be tried between 5×105-1×107;
2.Inoculated cells can be tried to be suspended with DMEM stock solution.
