B-hCD39 MC38

Basic Information

Common Name
B-hCD39 MC38
Aliases
ENTPD1
Catalog Number
311521
Cell Type/ Disease
Colon Carcinoma
Species/ Strain
Mus musculus, C57BL/6
Notice
Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines. Our humanized tumor cell lines are for pharmacology services ONLY.

Description

The mouse Entpd1 gene was replaced by human ENTPD1 coding sequence in B-hCD39 MC38 cells. Human CD39 is highly expressed on the surface of B-hCD39 MC38 cells.

Target strategy

The CMV promoter and human CD39 coding sequence were inserted into the targeting vector used in the lentivirus system.

Protein expression analysis

CD39 expression analysis in B-hCD39 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hCD39 MC38 cultures were stained with species-specific anti-CD39 antibody. Human CD39 was detected on the surface of B-hCD39 MC38 cells but not wild-type MC38 cells. The 1-A02 clone of B-hCD39 MC38 cells was used for in vivo experiments.

Tumor growth curve

Subcutaneous homograft tumor growth of B-hCD39 MC38 cells. B-hCD39 MC38 cells (1×106) and wild-type MC38 cells (1×106) were subcutaneously implanted into C57BL/6N mice (female, 8-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hCD39 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

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