B-hCD47 MC38 Plus

Basic Information

Common Name
B-hCD47 MC38 Plus
Aliases
IAP, MER6, OA3
Catalog Number
310696
Cell Type/ Disease
Colon Carcinoma
Species/ Strain
Mus musculus, C57BL/6
Notice
Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines. Our humanized tumor cell lines are for pharmacology services ONLY.

Target strategy

The IgV domain on exon 2 of mouse Cd47 gene was replaced by the IgV domain of human CD47 on exon 2 in B-hCD47 MC38 plus.

Protein expression analysis

CD47 expression analysis in B-hCD47 MC38 plus cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hCD47 MC38 plus cultures were stained with species-specific anti-CD47 antibody. Human CD47 was detected on the surface of B-hCD47 MC38 plus cells but not wild-type MC38 cells. The B2 clone of B-hCD47 MC38 plus cells was used for in vivo experiments.

Tumor growth curve

Subcutaneous homograft tumor growth of B-hCD47 MC38 plus cells. B-hCD47 MC38 plus cells (5×105) and wild-type MC38 cells (5×105) were subcutaneously implanted into B-hSIRPA/hCD47 mice (female, 9 week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hCD47 MC38 plus cells were able to establish tumors in vivo and can be used for efficacy studies.

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