B-hCXCR2 MC38

Basic Information

Common Name
B-hCXCR2 MC38
Aliases
CD182, CMKAR2, IL8R2, IL8RA, IL8RB
Catalog Number
310824
Cell Type/ Disease
Colon carcinoma
Species/ Strain
Mus musculus, C57BL/6
Notice
Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines.Our humanized tumor cell lines are for pharmacology services ONLY.

Description

The mouse Cxcr2 gene was replaced by human CXCR2 coding sequence in B-hCXCR2 MC38 cells. Human CXCR2 is highly expressed on the surface of B-hCXCR2 MC38 cells.

Targeting strategy

The exogenous promoter and human CXCR2 coding sequence was inserted to replace part of murine exon 2 ~ 3’UTR. The insertion disrupts the endogenous murine Cxcr2 gene, resulting in a non-functional transcript.

Protein expression analysis

CXCR2 expression analysis in B-hCXCR2 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hCXCR2 MC38 cultures were stained with species-specific anti-CXCR2 antibody. Human CXCR2 was detected on the surface of B-hCXCR2 MC38 cells but not wild-type MC38 cells. The 6-F02 clone of B-hCXCR2 MC38 cells was used for in vivo experiments.

Tumor growth curve

Subcutaneous homograft tumor growth of B-hCXCR2 MC38 cells. B-hCXCR2 MC38 cells (5×105) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6N mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hCXCR2 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

Back to top