Basic Information

Common Name
B-hCXCR5 MC38
Aliases
BLR1, CD185, MDR15
Catalog Number
311084
Cell Type/Disease
Colon carcinoma
Species/Strain
Mus musculus, C57BL/6
Application
B-hCXCR5 MC38 cells have the capability to establish tumors in vivo, which can be used for efficacy studies.
NCBI Gene ID

Description

The mouse Cxcr5 gene was replaced by human CXCR5 coding sequence in B-hCXCR5 MC38 cells. Human CXCR5 is highly expressed on the surface of B-hCXCR5 MC38 cells.

Targeting Strategy

Gene targeting strategy for B-hCXCR5 MC38 cells. The exogenous promoter and human CXCR5 coding sequence was inserted to replace part of murine exons 1~2. The insertion disrupts the endogenous murine Cxcr5 gene, resulting in a non-functional transcript.

Protein Expression Analysis

CXCR5 expression analysis in B-hCXCR5 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hCXCR5 MC38 cultures were stained with species-specific anti-CXCR5 antibody. Human CXCR5 was detected on the surface of B-hCXCR5 MC38 cells but not wild-type MC38 cells. The 2-A03 clone of B-hCXCR5 MC38 cells was used for in vivo experiments.

Tumor Growth Curve & Body Weight Changes

Subcutaneous homograft tumor growth of B-hCXCR5 MC38 cells. B-hCXCR5 MC38 cells (5×105) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6 mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hCXCR5 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

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