Basic Information

Common Name
B-hFGL1 MC38
HFREP1, HP-041, HPS, LFIRE1, fibrinogen like 1
Catalog Number
Cell Type/Disease
Colon carcinoma
Mus musculus, C57BL/6


The mouse Fgl1 gene was replaced by the human FGL1 coding sequence in B-hFGL1 MC38 cells. Human FGL1 is highly expressed and secreted by B-hFGL1 MC38 cells.


B-hFGL1 MC38 cells have the capability to establish tumors in vivo, which can be used for efficacy studies.

Targeting strategy

The exogenous promoter and human FGL1 coding sequence was inserted to replace part of murine exon 4 and all of exons 5-7. The insertion disrupts the endogenous murine Fgl1 gene, resulting in a non-functional transcript.

Protein Expression Analysis


FGL1 expression analysis in B-hFGL1 MC38 cells by ELISA. Supernatant was collected from wild-type MC38 and B-hFGL1 MC38 cultures and analyzed using a species-specific FGL1 ELISA kit. Human FGL1 was detectable in the supernatant of B-hFGL1 MC38 cells but not in that of wild-type MC38 cells. The 3-A07 clone of B-hFGL1 MC38 cells was used for in vivo experiments.

Tumor Growth Curve

Tumor Growth Curve & Body Weight Changes in C57BL/6N Mice

Subcutaneous homograft tumor growth of B-hFGL1 MC38 cells. B-hFGL1 MC38 cells (5×105) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6N mice (female, 8-week-old, n=6). (A) Average tumor volume ± SEM and (B) body weight (mean ± SEM) were measured twice a week. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hFGL1 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

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