Basic Information

Common Name
B-hGPC3 MC38
Aliases
GPC3, DGSX, GTR2-2, MXR7, OCI-5, SDYS, SGB, SGBS, SGBS1, glypican 3
Catalog Number
311576
Cell Type/Disease
Colon carcinoma
Species/Strain
Mus musculus, C57BL/6
NCBI Gene ID

Description

The mouse Gpc3 gene was replaced by the human GPC3 coding sequence in B-hGPC3 MC38 cells. Human GPC3 is highly expressed on the surface of B-hGPC3 MC38 cells.

Application

B-hGPC3 MC38 cells have the capability to establish tumors in vivo, which can be used for efficacy studies.

Targeting strategy

The exogenous promoter and human GPC3 coding sequence was inserted to replace part of murine exon 1. The insertion disrupts the endogenous murine Gpc3 gene.

Protein Expression Analysis

Pre-inoculation

GPC3 expression analysis in B-hGPC3 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hGPC3 MC38 cultures were stained with species-specific anti-GPC3 antibody. Human GPC3 was detected on the surface of B-hGPC3 MC38 cells but not wild-type MC38 cells. The 1-E12 clone of B-hGPC3 MC38 cells was used for in vivo tumor growth assays.

Post-inoculation

B-hGPC3 MC38 cells were subcutaneously transplanted into heterozygous B-hGPC3 mice (n=5). Tumor cells were harvested on day 31 post inoculation, and assessed for human GPC3 expression by flow cytometry. As shown, human GPC3 was highly expressed on the surface of humanized B-hGPC3 MC38 tumor cells, indicating B-hGPC3 MC38 cells can be used for in vivo efficacy studies of GPC3 therapeutics.

Tumor Growth Curve

Tumor Growth Curve & Body Weight Changes in Humanized Mice

Subcutaneous homograft tumor growth of B-hGPC3 MC38 cells. B-hGPC3 MC38 cells (5×105) and wild-type MC38 cells (5×105) were subcutaneously implanted into heterozygous B-hGPC3 mice (female, 5-week-old, n=5). (A) Average tumor volume ± SEM and (B) body weight (mean ± SEM) were measured twice a week. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hGPC3 MC38 cells were able to form tumors in vivo, which can be used for efficacy studies. Note that B-hGPC3 MC38 cells successfully formed tumors only in heterozygous B-hGPC3 mice, but not in wild-type mice.