Basic Information

Common Name
B-hSIGLEC15 MC38
Aliases
CD33L3, HsT1361, SIGLEC-15
Catalog Number
311049
Cell Type/ Disease
Colon carcinoma
Species/ Strain
Mus musculus, C57BL/6
Notice
Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines.Our humanized tumor cell lines are for pharmacology services ONLY.

Description

The mouse Siglec15 gene was replaced by human SIGLEC15 coding sequence in B-hSIGLEC15 MC38 cells. Human SIGLEC15 is highly expressed on the surface of B-hSIGLEC15 MC38 cells.

Targeting strategy

The exogenous promoter and human SIGLEC15 coding sequence was inserted to replace part of murine exon 3 ~ 3’UTR. The insertion disrupts the endogenous murine Siglec15 gene, resulting in a non-functional transcript.

Protein expression analysis

SIGLEC15 expression analysis in B-hSIGLEC15 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hSIGLEC15 MC38 cultures were stained with species-specific anti-SIGLEC15 antibody. Human SIGLEC15 was detected on the surface of B-hSIGLEC15 MC38 cells but not wild-type MC38 cells. The 3-D10 clone of B-hSIGLEC15 MC38 cells was used for in vivo experiments.

Subcutaneous homograft tumor growth of B-hSIGLEC15 MC38 cells. B-hSIGLEC15 MC38 cells (5×105) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6N mice (female, 5-8-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hSIGLEC15 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

Recommendations for inoculation in other mouse strains:

1.Cell inoculation amount is recommended to be tried between 5×105-1×107;

2.Inoculated cells can be tried to be suspended with DMEM stock solution.