Basic Information
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Targeting Strategy
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Details
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Cell protein expression analysis
B-hTNFR2 MC38 Cell Line were analyzed by flow cytometry.
Result: B-hTNFR2 MC38 Cell Line expressed human TNFR2 protein, but not mouse TNFR2 protein.
Tumor growth curve
B-hTNFR2 MC38 cells were subcutaneously implanted into the C57BL/6N mice. Tumor size and mice body weight were measured twice a week. Volume was expressed in mm3 using the formula: V=0.5a X b2 where a and b were the long and short diameters of the tumor, respectively(n=5). (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.
Results: B-hTNFR2 MC38 cells were able to establish tumor model steadily and can effectively track tumor volume.
Tumor expression detection sample information
Protein expression analysis in tumor cells
Note:
FMO Control 1: MC38-Tumor unstained mTNFR2 PE antibody;
FMO Control 2: MC38-Cells unstained mTNFR2 PE antibody.
Protein expression analysis in tumor cells
Note:
FMO Control 1: MC38-Tumor unstained mTNFR2 PE antibody;
FMO Control 2: MC38-Cells unstained mTNFR2 PE antibody.
B-hTNFR2 MC38 was transplanted into C57BL/6N mice(n=5), and on 27 days post inoculation, certain volumes of tumors as was taken to detect the expression level of the cells.
Results: hTNFR2 was highly expressed on the surface of tumor cells.