Basic Information

Common Name
B-hTPBG MC38
Aliases
5T4
Catalog Number
311068
Cell Type/ Disease
Colon carcinoma
Species/ Strain
Mus musculus, C57BL/6
Notice
Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines.Our humanized tumor cell lines are for pharmacology services ONLY.

Description

The mouse Tpbg gene was replaced by human TPBG coding sequence in B-hTPBG MC38 cells. Human TPBG is highly expressed on the surface of B-hTPBG MC38 cells.

Targeting strategy

The exogenous promoter and human TPBG coding sequence was inserted to replace part of murine exon 2. The insertion disrupts the endogenous murine Tpbg gene, resulting in a non-functional transcript.

Protein expression analysis

TPBG expression analysis in B-hTPBG MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hTPBG MC38 cultures were stained with species-specific anti-TPBG antibody. Human TPBG was detected on the surface of B-hTPBG MC38 cells but not wild-type MC38 cells. The 2-C06 clone of B-hTPBG MC38 cells was used for in vivo experiments.

Tumor growth curve

Subcutaneous homograft tumor growth of B-hTPBG MC38 cells. B-hTPBG MC38 cells (5×105) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6N mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hTPBG MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.