B-mSiglec15 MC38

Basic Information

Common Name
B-mSiglec15 MC38
Aliases
Cd33l, Cd33l3, EG620235, SIGLEC-I
Catalog Number
311529
Cell Type/ Disease
Colon carcinoma
Species/ Strain
Mus musculus, C57BL/6
Notice
Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines.Our humanized tumor cell lines are for pharmacology services ONLY.

Description

The mouse Siglec15 gene was insert into mouse ROSA26 site. Mouse Siglec15 is highly expressed on the surface of B-mSiglec15 MC38 cells.

Targeting strategy

The mouse Siglec15 CDS was insert into mouse ROSA26 site in B-mSiglec15 MC38.

Protein expression analysis

The Siglec15 expression analysis in B-mSiglec15 MC38 cells by flow cytometry. Single cells were collected from wild-type MC38 and B-mSiglec15 MC38 cells, and analyzed by flow cytometry with anti-human and mouse SIGLEC15 antibody(SIGLEC15-5G12, in house). Mouse Siglec15 was exclusively detectable in B-mSiglec15 MC38 cells but not wild-type MC38 cells. The 2-C12 clone of B-mSiglec15 MC38 cells was used for in vivo experiments.

Tumor growth curve

Subcutaneous homograft tumor growth of B-mSiglec15 MC38 cells. B-mSiglec15 MC38 cells (5×105) and wild-type MC38 cells (5×105 ) were subcutaneously implanted into the C57BL/6N mice (female, 5-8 week-old, n=5).Tumor size and mice body weight were measured twice a week. (A) Tumor average volume ± SEM, (B) Mice body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5a X b2, where a and b were the long and short diameters of the tumor, respectively. As shown in panel A, B-mSiglec15 MC38 were able to establish tumor in vivo and can be used for efficacy studies.

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