Basic Information
Strain Name
C57BL/6-Col1a1tm1(Alb-iCreERT2)/Bcgen
Common Name
B-Alb-iCreERT2 mice
Background
C57BL/6
Catalog Number
110137
Application
B-Alb-iCreERT2 mice expressing icreERT2 under the control of the Alb promoter allow efficient Tamoxifen-dependent recombination of floxed DNA selectively in hepatocytes.
Related Genes
Alb, albumin; Alb1; Alb-1; BCL002
Description
Homozygous mice for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
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Phenotype Analysis
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- Analysis of liver by immunofluorescence (IF).
Control: without tamoxifen treatment; HNF4α: a regulator of liver-specific gene expression.
Immunofluorescence for HNF4α, tdTomato, and DAPI on liver sections shows Alb-iCreERT2(Mut/+), CAG-LSL-tdTomato(Mut/+) mice labeled almost all hepatocytes after tamoxifen treatment.
- Analysis of kidney by immunofluorescence (IF).
Control: without tamoxifen treatment; HNF4α: a regulator of liver-specific gene expression.
Immunofluorescence for HNF4α, tdTomato, and DAPI on kidney sections shows Alb-iCreERT2(Mut/+), CAG-LSL-tdTomato(Mut/+) mice were not labeled after tamoxifen treatment.
