Basic Information

Strain Name
C57BL/6N-Slamf1tm1(icre/ERT2)Bcgen/Bcgen
Stock Number
110142
Common Name
B-CD150-iCreERT2 mice
Related Genes
Pdgfrb (platelet derived growth factor receptor, beta polypeptide); CD140b
Background
C57BL/6N
Appearance
Black
Development
A construct including the P2A-icreERT2 was inserted between the coding sequence of exon7 and 3’UTR of the CD150 gene. These mice were maintained on a C57BL/6N background.
NCBI Gene ID
Application
B-CD150-iCreERT2 mice expressing icreERT2 under the control of the CD150 promoter allow efficient Tamoxifen-dependent recombination of floxed DNA selectively in CD150+ cells.

Description

Homozygous null mice are fertile and display impaired T cell and macrophage cytokine production.

Targeting Strategy

Phenotype Analysis

The B-CD150-iCreERT2 mice were crossed with B-CAG-tdTomato cKI mice (stock no. 110148) to obtain heterozygous B-CD150-iCreERT2; tdTomato mice. Blood cells were collected from wild-type C57BL/6 mice, heterozygous B-CAG-tdTomato cKI mice and heterozygous B-CD150-iCreERT2; tdTomato mice after 48 h i.p. injected with tamoxifen (each mouse is injected with 100μL of tamoxifen, dissolved in corn oil, at a concentration of 20 mg/mL) for five consecution days and analyzed by flow cytometry. The flow cytometry results showed that the expression of tdTomato fluorescent protein was not detected in the immune cells of wild-type mice, B-CAG-tdTomato cKI mice, and B-CD150-iCreERT2; tdTomato mice without tamoxifen induction (Fig. A and B). After tamoxifen induction, the expression of tdTomato fluorescent protein was not detected in the immune cells of wild-type and B-CAG-tdTomato cKI mice. However, the expression of tdTomato fluorescent protein was detected in the neutrophils and macrophages of anticoagulant cells in B-CD150-iCreERT2; tdTomato mice; no detection was found in unactivated B cells, T cells, NK cells, and monocytes. (Fig. C and D).

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