Basic Information

Strain Name
C57BL/6-Cx3cr1tm1(icre/ERT,EGFP)Bcgen/Bcgen
Stock Number
110779
Common Name
B-Cx3cr1-iCreERT2-EGFP mice
Gene symbol and name
Cx3cr1,chemokine (C-X3-C motif) receptor 1
Background
C57BL/6N
Coat color
Black
Application
  • Function research of genes
  • Function research of microglial cells and other Cx3cr1-expressing immune cells
NCBI Gene ID
Development
A targeting vector was designed to replace 390bp of exon 2 of the Cx3cr1 gene with a iCre/ERT2 (improved cre recombinase fulsed to estrogen receptor 1) coding sequence, followed by a porcine teschovirus-1 2A (P2A) and a enhanced green fluorescent protein(EGFP). The targeting vector were injected into C57BL/6N mouse zygotes. After injection, surviving 2-cell-stage zygotes were transplanted to the KM albino pseudopregnant females. The reslulting Cx3cr1-iCreERT2-EGFP founder mice were bred to C57BL/6N mice. These mice were maintained on a C57BL/6N background.

Description

Homozygous mice for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. In this strain, iCre/ERT2 fusion protein and EGFP expression is under the control of Cx3cr1 promoter. When crossed with a strain containing a loxP-site flanked sequence of interest, Cre-mediated recombination will result in deletion of the floxed sequence in the offspring.

Gene editing strategy

A targeting vector was designed to replace 390bp of exon 2 of the Cx3cr1 gene with a iCre/ERT2 (improved cre recombinase fulsed to estrogen receptor 1) coding sequence, followed by a porcine teschovirus-1 2A (P2A) and a enhanced green fluorescent protein (EGFP). The targeting vector were injected into C57BL/6N mouse zygotes. After injection, surviving 2-cell-stage zygotes were transplanted to the KM albino pseudopregnant females. The reslulting Cx3cr1-iCreERT2-EGFP founder mice were bred to C57BL/6N mice. These mice were maintained on a C57BL/6N background.

Phenotype analysis

The expression of EGFP and tdTomato in mouse spleen, bone marrow, and brain mononuclear macrophages was detected by flow cytometry, and Cx3cr1 (Mut /+) was found after induction of Tamoxifen.The majority of macrophages in the brain of tdTomato (Mut/+) mice were double positive cells of EGFP and tdTomato, while the mononuclear macrophages in spleen and bone marrow were single positive cells of EGFP. The results were consistent with the results in the study, indicating that B-Cx3cr1-iCreERT2-EGFP mice were designed and expressed successfully.

Publications using B-Cx3cr1-iCreERT2-EGFP mice

Wang R, Liang Z, Xue X, Mei H, Ji L, Wang B, Chen W, Gao C, Yuan S, Wu T, Qi H, Hu S, Yi L, Song Y, Liao R, Chen B. Microglial FoxO3a deficiency ameliorates ferroptosis-induced brain injury of intracerebral haemorrhage via regulating autophagy and heme oxygenase-1. J Cell Mol Med. 2023 Oct 27. doi: 10.1111/jcmm.18007. Epub ahead of print. PMID: 37890842.

References

  1. GoldmannTobias,WieghoferPeter,Müller Philippe F et al. A new type of microglia gene targeting shows TAK1 to be pivotal in CNS autoimmune inflammation.[J] .Nat. Neurosci., 2013, 16: 1618-26. [PMID: 24077561]
  2. YonaSimon,Kim Ki-Wook,Wolf Yochai et al. Fate mapping reveals origins and dynamics of monocytes and tissue macrophages under homeostasis.[J] .Immunity, 2013, 38: 79-91. [PMID: 23273845]
  3. Rogers J T ,Morganti J M , Bachstetter A D , et al. CX3CR1 Deficiency Leads to Impairment of Hippocampal Cognitive Function and Synaptic Plasticity[J]. Journal of Neuroscience, 2011, 31(45):16241-16250. [PMID: 22072675]
  4. Ushijima T , Fujimoto N , Matsuyama S , et al. The actin-organizing formin protein Fhod3 is required for postnatal development and functional maintenance of the adult heart in mice[J]. Journal of Biological Chemistry, 2017:jbc.M117.813931. [PMID: 29158260]
  5. Madisen L ,Zwingman T A , Sunkin S M , et al. A robust and high-throughput Cre reporting and characterization system for the whole mouse brain[J]. NATURE NEUROSCIENCE, 2009, 13(1):133-140. [PMID: 20023653]
Back to top