Dcp2 encoded by Dcp2 gene. It is a key component of an mRNA-decapping complex required for degradation of mRNAs, both in normal mRNA turnover, and in nonsense-mediated mRNA decay (NMD). It removes the 7-methyl guanine cap structure from mRNA, prior to its degradation from the 5′ end. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Primer Sequence (5’-3’) Tm (℃) Product Size (bp) Primer-WT-F GAGTGCGTGAAAGGCAATTTCTGGT 63 Mut:444 Primer -Mut-R CACACAGACAGGAGCATCTTCCAGG 63 Primer -WT-F GAGTGCGTGAAAGGCAATTTCTGGT 63 WT:353
Primer -WT-R CTTTAGAAACATGGCCTCCCTGCAC 63
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