Basic Information
Strain Name
C57BL/6-Gfaptm1(icre)/Bcgen
Stock Number
110144
Common Name
B-Gfap-iCre mice
Source/Investigator
Biocytogen Jiangsu Co., Ltd
Related Genes
Gfap (glial fibrillary acidic protein)
Species
C57BL/6
Appearance
Black
Genotype
Gfap iCre (wt/mut)
Development
An iCre-WPRE-pA cassette was placed at the 5’ end of the Gfap gene using C57BL/6 embryonic stem cells. Neo cassette flanked by frt sites was inserted at downstream of pA. This strain was maintained on a C57BL/6 genetic background.
Description
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
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Targeting Strategy
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Details
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Genotyping Information
Primer Sequence (5’-3’) Tm (℃) Product Size (bp) 110155-WT-F GTCAGGGGCAGATTTAGTCCAACCC 58 WT:194bp 110155-WT-R TCATTCGAGACAAGGAGAAGCGTGG 58 110155-WT-F GTCAGGGGCAGATTTAGTCCAACCC 58 Mut:247bp 110155-Mut-R GCACACAGACAGGAGCATCTTC 57 -
References
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1. The design for ES-GJ-020tm1Mes is based on McCall MA, et al., Targeted deletion in astrocyte intermediate filament (ES-GJ-020) alters neuronal physiology. Proc Natl Acad Sci U S A. 1996 Jun 25;93(13):6361-6.
