Basic Information

Strain Name
C57BL/6-Gfaptm1(icre)/Bcgen
Stock Number
110144
Common Name
B-Gfap-iCre mice
Source/Investigator
Biocytogen Jiangsu Co., Ltd
Related Genes
Gfap (glial fibrillary acidic protein)
NCBI Gene ID
Species
C57BL/6J
Appearance
Black
Genotype
Gfap iCre (wt/mut)
Development
An iCre-WPRE-pA cassette was placed at the 5’ end of the Gfap gene using C57BL/6 embryonic stem cells. Neo cassette flanked by frt sites was inserted at  downstream of pA. This strain was maintained on a C57BL/6 genetic background.
Application
This Gfap-iCre model is an efficient tool to study various gene functions when crossed with mice with different loxP site-flanked genes of interest, especially in studies of astrocytes cells related signaling.

Gene editing strategy

A target vector was designed to insert iCre-WPRE-PA cassette into mouse endogenous genes 5 ‘UTR downstream of GFAP. The targeting vector was electroporated into C57BL/6-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulted chimeric mice. These mice were maintained on a C57BL/6 background.

Phenotype analysis

In situ hybridization in hippocampal CA1 area to confirm µR selective deletion from µRGFAP-/- mice with probes of µR mRNA (red), GAD2/ GFAP/vGlut1 mRNA (green), and DAPI nucleus staining (blue).

Reference

1. McCall MA, Gregg RG, Behringer RR, Brenner M, Delaney CL, Galbreath EJ, Zhang CL, Pearce RA, Chiu SY, Messing A. Targeted deletion in astrocyte intermediate filament (Gfap) alters neuronal physiology. Proc Natl Acad Sci U S A. 1996 Jun 25;93(13):6361-6. [PubMed: 8692820]