Basic Information

Strain Name
C57BL/6-C5ar2tm1(C5AR2)/Bcgen
Common Name
B-hC5AR2 mice
Background
C57BL/6
Catalog number
111001
Aliases
C5L2, GPF77, GPR77
NCBI Gene ID

mRNA expression analysis

Strain specific analysis of C5AR2 gene expression in wild-type C57BL/6 mice and B-hC5AR2 mice by RT-PCR. Mouse C5ar2 mRNA was detectable in spleen of wild-type C57BL/6 mice (+/+) but not in homozygous B-hC5AR2 mice (H/H). Human C5AR2 mRNA was detectable only in homozygous B-hC5AR2 mice but not in wild-type mice.

Protein expression analysis

Strain specific C5AR2 expression analysis in homozygous B-hC5AR2 mice by FACS. Peritoneal washes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hC5AR2 mice (H/H) and analyzed by FACS with anti-C5AR2 antibody. C5AR2 was both detectable in wild-type mice and homozygous B-hC5AR2 mice with anti-mC5AR2 antibody. Anti-mC5AR2 antibody was cross-reactive between mouse and human (data not shown).

Summary

mRNA expression analysis:

Mouse C5ar2 mRNA was detectable in spleen of wild-type C57BL/6 mice. Human C5AR2 mRNA was detectable only in homozygous B-hC5AR2 mice.

Protein expression analysis:

C5AR2 was both detectable in wild-type mice and homozygous B-hC5AR2 mice with anti-mC5AR2 antibody. This antibody was cross-reactive between mouse and human (data not shown).