Basic Information
Description
The Ncr1 gene, also called Nkp46, encodes the natural cytotoxicity triggering receptor 1. This protein is expressed specifically in natural killer (NK) cells and possibly in related innate lymphocytes. In this strain, Cre recombinase expression is under the control of Ncr1 promoter. When crossed with a strain containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked sequence in Ncr1 expressing cells.
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Targeting Strategy
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Details
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Genotyping Information
Primer Sequence (5’-3’) Tm
(℃)Product size
(bp)Ncr1 iCre primer 1 CGAATTGGTCTGGCATGCATAATC 58 WT: 285 Ncr1 iCre primer 2 CTTTGCCAAACTTGGTAACACTCC 58 Ncr1 iCre primer 1 CGAATTGGTCTGGCATGCATAATC 58 Mut: 282 Ncr1 iCre primer 3 CACACCGGCCTTATTCCAAG 57 Polymerase: Taq
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References
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1. Chaushu S; Wilensky A; Gur C; Shapira L; Elboim M; Halftek G; Polak D; Achdout H;
Bachrach G; Mandelboim O. 2012. Direct recognition of Fusobacterium nucleatum by the NK cell natural cytotoxicity receptor NKp46 aggravates periodontal disease. PLoS Pathog 8(3):e1002601. [PubMed:22457623]
2. Elboim M; Gazit R; Gur C; Ghadially H; Betser-Cohen G; Mandelboim O. 2010. Tumor
immunoediting by NKp46. J Immunol 184(10):5637-44. [PubMed:20404273]
3. Ghadially H; Horani A; Glasner A; Elboim M; Gazit R; Shoseyov D; Mandelboim O. 2013. NKp46 regulates allergic responses. Eur J Immunol 43(11):3006-16. [PubMed: 23878025]
4. Ghadially H; Ohana M; Elboim M; Gazit R; Gur C; Nagler A; Mandelboim O. 2014. NK Cell Receptor NKp46 Regulates Graft-versus-Host Disease. Cell Rep 7(6):1809-14. [PubMed: 24882008]
5. Glasner A; Ghadially H; Gur C; Stanietsky N; Tsukerman P; Enk J; Mandelboim O. 2012.
Recognition and Prevention of Tumor Metastasis by the NK Receptor NKp46/NCR1. J Immunol 188(6):2509-15. [PubMed: 22308311]
6. Gur C; Porgador A; Elboim M; Gazit R; Mizrahi S; Stern-Ginossar N; Achdout H; Ghadially H; Dor Y; Nir T; Doviner V; Hershkovitz O; Mendelson M; Naparstek Y; Mandelboim O. 2010. The activating receptor NKp46 is essential for the development of type 1 diabetes. Nat Immunol 11(2):121-8. [PubMed: 20023661]
