Apolipoprotein A4 is a plasma protein and synthesized in the intestine and liver. ApoA4 is secreted into circulation on the surface of newly synthesized chylomicron particles. The primary functions of ApoA4 have not been clearly established; however, ApoA4 has been implicated in regulating appetite and satiety in rodent models, modulating the lipid transport in vitro, and activating lecithin-cholesterol acyltransferase and cholesterylester transfer protein in vitro.
Primer Sequence (5’-3’) Tm (℃ Product size (bp) Apoa4KO primer 1 GACGCTAGGAAACATCCAGTGTAGC 62 Mut: 694
Apoa4KO primer 2 CCATTTACCAAACGCTAGCACATGC 62
The fragments need to be verified by sequencing.
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