C57BL/6-F8 tm1 /Bcgen
B-F8 KO mice
Bcgen (Beijing Biocytogen Co., Ltd)
F8；FVIII（coagulation factor VIII）
The B-F8 KO mice were developed by Beijing Biocytogen Co., Ltd. The Exons 16-20 of mice were knocked out.
NCBI Gene ID
Primer Sequence (5’-3’) Tm (℃) Product size
F8-WT-F ACTGTGGCTGGGTGTAGTAGCAC 63 WT: 356 F8-WT-R TTTCTCCAAGTTGCAGGTCAAGGCT 60 F8-WT-F ACTGTGGCTGGGTGTAGTAGCAC 63 Mut: ~400
F8-Mut-R CGCATAATACACCCTGGTCACACTCAC 63
NovoSeven efficacy evaluation
Animals were randomly divided into Control group or NovoSeven (1 mg/kg), After 30 minutes treatment, the abdominal aorta was collected blood for ATPP (activated partial thromboplastin time) detection. The results showed that the APTT recovered to normal after drug treatment compared with the control group.
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2. Blood. 2014 Jun 12;123(24):3706-13. doi: 10.1182/blood-2014-02-555151. Epub
2014 Apr 4.