B-F8 KO mice

Basic Information

Targeting Strategy

Model validation analysis

The experimental animals were randomly divided into groups and given NS(normal saline) or 1 mg/kg NovoSeven by tail vein injection, 30 minutes after injection of the drug, blood was collected from the abdominal aorta to detect the blood coagulation index: activated partial thromboplastin time APTT.

The results showed that APTT values in F8 KO mice were much higher than those in WT mice, and APTT returned to normal values after injection of NovoSeven (recombinant human coagulation factor VIIa).

The results showed that B-F8 KO mice can be used as a powerful tool for the validation of anticoagulant efficacy.

Details

Genotyping Information

Primer	Sequence (5’-3’)	Tm (℃)	Product size （bp）
F8-WT-F	ACTGTGGCTGGGTGTAGTAGCAC	63	WT: 356
F8-WT-R	TTTCTCCAAGTTGCAGGTCAAGGCT	60
F8-WT-F	ACTGTGGCTGGGTGTAGTAGCAC	63	Mut: ~400 WT: 5925
F8-Mut-R	CGCATAATACACCCTGGTCACACTCAC	63

Polymerase: Taq

Application

NovoSeven efficacy evaluation

Animals were randomly divided into Control group or NovoSeven (1 mg/kg), After 30 minutes treatment, the abdominal aorta was collected blood for ATPP (activated partial thromboplastin time) detection. The results showed that the APTT recovered to normal after drug treatment compared with the control group.

References

1. Nat Genet. 1995 May;10(1):119-21.
2. Blood. 2014 Jun 12;123(24):3706-13. doi: 10.1182/blood-2014-02-555151. Epub
2014 Apr 4.