This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X- linked coagulation disorder.
Primer Sequence (5’-3’) Tm (℃) Product size
F8-WT-F ACTGTGGCTGGGTGTAGTAGCAC 63 WT: 356 F8-WT-R TTTCTCCAAGTTGCAGGTCAAGGCT 60 F8-WT-F ACTGTGGCTGGGTGTAGTAGCAC 63 Mut: ~400
F8-Mut-R CGCATAATACACCCTGGTCACACTCAC 63
NovoSeven efficacy evaluation
Animals were randomly divided into Control group or NovoSeven (1 mg/kg), After 30 minutes treatment, the abdominal aorta was collected blood for ATPP (activated partial thromboplastin time) detection. The results showed that the APTT recovered to normal after drug treatment compared with the control group.
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2. Blood. 2014 Jun 12;123(24):3706-13. doi: 10.1182/blood-2014-02-555151. Epub
2014 Apr 4.