C57BL/6-F9 tm1 /Bcgen
B-F9 KO mice
Bcgen (Beijing Biocytogen Co., Ltd)
F9；FIX（coagulation factor IX）
The B-F9 KO mice were developed by Beijing Biocytogen Co., Ltd. The Exons 1-3 of mice were knocked out.
NCBI Gene ID
Primer Sequence (5’-3’) Tm
63 WT: 7049
F9-Mut-R2 GAATTGGACCTCAGGTAGATGGAGC 63 F9-WT-F3 AACACGTTGCAGAAAGATCCTGTCA 63 WT: 474 F9-WT-R3 AAGTCAGCCTGTGAAATGTCCCTCA 64
NovoSeven efficacy evaluation
Animals were randomly divided into Control group or NovoSeven (1 mg/kg), After 30 minutes treatment, the abdominal aorta was collected blood for ATPP (activated partial thromboplastin time) and FIB (Fibrinogen) detection. The results showed that the APTT recovered to normal after drug treatment compared with the control group. FIB values were not significantly different from WT mice. A:ATPP±SEM; B: FIB±SEM.
1. Blood. 1998 Jul 1;92(1):168-74
2. Proc Natl Acad Sci U S A. 1997 Oct 14;94(21):11563-6.