The Il2rg KO rat was developed using targeted TALEN technology in the Sprague-Dawley strain. This model possesses a 14 bp deletion within Exon 2 of IL2rg on chromosome X. This strain was maintained by mating homozygous males with homozygous females on a Sprague-Dawley genetic background.
Primer Sequence (5’-3’) Tm
IL2rg KO primer 1 GCTCCAAGGTCCTCATGTCCAG 62 Mut: 915
IL2rg KO primer 2 ATCTTCGCCTTTCTGCCCATGAC 62
The fragments need to be verified by sequencing.
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