Il2rg is known as the interleukin receptor common gamma chain, which is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21. Mutations in this gene cause X-linked severe combined immunodeficiency.
Primer Sequence (5’-3’) Tm
IL2rg KO primer 1 GCTCCAAGGTCCTCATGTCCAG 62 Mut: 915
IL2rg KO primer 2 ATCTTCGCCTTTCTGCCCATGAC 62
The fragments need to be verified by sequencing.
- Ohbo, K., et al., Modulation of hematopoiesis in mice with a truncated mutant of the interleukin-2 receptor gamma chain. Blood, 1996. 87(3): p. 956-67. [PubMed: 8562967]
- Mashimo, T., et al., Generation of knockout rats with X-linked severe combined
immunodeficiency (X-SCID) using zinc-finger nucleases. PLoS One, 2010. 5(1): p. e8870.
- Mashimo, T., et al., Generation and characterization of severe combined immunodeficiency rats. Cell Rep, 2012. 2(3): p. 685-94. [PubMed: 22981234]