Basic Information

Strain name
C57BL/6N-Abcb1atm1BcgenAbcb1btm1Bcgen/Bcgen 
Common Name
B-Mdr1 KO mice
Background
C57BL/6N
Catalog Number
112683
Aliases
CLCS; MDR1; P-GP; PGY1; ABC20; CD243; GP170; Abcb1a,Abcb1b

Targeting strategy

Gene targeting strategy for B-Mdr1 KO mice. The genome sequences between mouse Mdr1a gene and Mdr1b gene were depleted in B-Mdr1 KO mice. As a result, mouse MDR1 protein will be not expressed anymore.

mRNA expression analysis

Mdr1a mRNA expression analysis in wild-type C57BL/6 mice and B-Mdr1 KO mice by RT-PCR. Brain, lung and liver RNA were isolated from wild-type C57BL/6 mice and B-Mdr1 KO mice, then cDNA libraries were synthesized by reverse transcription, followed by PCR with two mouse Mdr1a primer pairs. Mouse Mdr1a mRNA was not detectable in Mdr-1 knock-out mice.

Mdr1b mRNA expression analysis in wild-type C57BL/6 mice and B-Mdr1 KO mice by RT-PCR. Brain, lung and liver RNA were isolated from wild-type C57BL/6 mice and B-Mdr1 KO mice, then cDNA libraries were synthesized by reverse transcription, followed by PCR with two mouse Mdr1b primer pairs. Mouse Mdr1b mRNA was not detectable in Mdr-1 knock-out mice.

Protein expression analysis

Western blot analysis of MDR1 protein expression in B-Mdr1 KO mice. Various tissue lysates were collected from wild-type C57BL/6 mice and B-Mdr1 KO mice, and then analyzed by western blot with anti-MDR1A antibody. 40μg total proteins were loaded for western blotting analysis. MDR1 was only detectable in adrenal, brain, kidney and lung from wild-type mice but not in Mdr-1 knock-out mice.

Summary

  • mRNA expression analysis:

Mouse Mdr1a and Mdr1b were not detectable in brain, lung and liver from Mdr-1 knock-out mice. 

  • Protein expression analysis:

Mouse MDR1 was detectable in adrenal, brain, kidney and lung from wild-type mice, but not in Mdr-1 knock-out mice.

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