Basic Information

Strain Name
C57BL/6-Trp53 tm1 /Bcgen
Stock Number
110167
Common Name
B-p53 KO mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
p53; Trp53
Species
C57BL/6
Appearance
Black
Genotypes
Trp53 (mut/mut); Trp53 (mut/wt)
Development
The Trp53- mice were developed by Beijing Biocytogen Co., Ltd. The targeted mouse Trp53 exons 2-7 were replaced with pGK-Neo cassette resulting in inactivation of Trp53 protein. The C57BL/6 ES cell line was used.

Description

The Trp53 gene encodes tumor protein p53, which involves in diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. P53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines. Mice with homozygous p53 inactivation are developmentally normal but are susceptible to spontaneous tumors.

Targeting Strategy

Details

Genotyping Information

Primer Sequence (5’-3’) Tm
(℃)
Product size
(bp)
Trp53 primer 1 AGTTCTGCCACGTGGTTGGT 60 WT: 281
Trp53 primer 2 GTCTCCTGGCTCAGAGGGAG 60
Trp53 primer 1 AGTTCTGCCACGTGGTTGGT 60 Mut: 441
Trp53 primer 3 CAGAGGCCACTTGTGTAGCG 60

Polymerase: Taq

 

 

 

 

Publications

  1. Silencing of functional p53 attenuates NAFLD by promoting HMGB1-related autophagy induction
  2. Olaquindox-Induced Liver Damage Involved the Crosstalk of Oxidative Stress and p53 In Vivo and In Vitro
  3. SIRT6 as a key event linking P53 and NRF2 counteracts APAP-induced hepatotoxicity through inhibiting oxidative stress and promoting hepatocyte proliferation
  4. p53-mediated control of aspartate-asparagine homeostasis dictates LKB1 activity and modulates cell survival
  5. Mutant p53 Drives Cancer Metastasis via RCP-Mediated Hsp90α Secretion
  6. Heavy ion radiation-induced DNA damage mediates apoptosis via the Rpl27a-Rpl5-MDM2-p53/E2F1 signaling pathway in mouse spermatogonia
  7. Deficiency in the anti-apoptotic protein DJ-1 promotes intestinal epithelial cell apoptosis and aggravates inflammatory bowel disease via p53

References

1. Jacks T; Remington L; Williams BO; Schmitt EM; Halachmi S; Bronson RT; Weinberg RA. 1994. Tumor spectrum analysis in p53-mutant mice. Curr Biol 4(1):1-7. [PMID: 7922305]

2. Shao C; Deng L; Henegariu O; Liang L; Stambrook PJ; Tischfield JA. 2000. Chromosome instability contributes to loss of heterozygosity in mice lacking p53. Proc Natl Acad Sci U S A 97(13):7405-10. [PMID: 10861008]