Basic Information
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Targeting strategy
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Gene targeting strategy for B-hAFP mice. The full-length protein of human AFP CDS were inserted into the mouse exon2.
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mRNA and protein expression analysis
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Strain specific analysis of AFP gene expression in wild-type mice and B-hAFP mice by RT-PCR. Mouse Afp mRNA was detectable in fetal liver of wild-type mice (+/+). Human AFP mRNA was detectable only in homozygous B-hAFP mice (H/H) but not in wild-type mice.
Strain specific AFP expression analysis in homozygous B-hAFP mice by western blot. Fetal liver tissue were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hAFP mice (H/H), and analyzed by western blot with anti-hAFP antibody. Mouse AFP was detectable in wild-type mice. Human AFP was only detectable in homozygous B-hAFP mice but not in wild-type mice.
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Summary
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mRNA and protein expression analysis:
Human AFP mRNA was detectable in B-hAFP mice, and we proved that the protein could be expressed normally by western blot analysis.
