Basic Information

Strain name
C57BL/6NAfptm1(AFP)/Bcgen
Common Name
B-hAFP mice
Background
C57BL/6N
Catalog Number
111622
Aliases
AFP (AFPD, FETA, HPAFP)
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hAFP mice.

The full-length protein of human AFP CDS were inserted into the mouse exon2.

mRNA and protein expression analysis

Strain specific analysis of AFP gene expression in wild-type mice and B-hAFP mice by RT-PCR. Mouse Afp mRNA was detectable in fetal liver of wild-type mice (+/+). Human AFP mRNA was detectable only in homozygous B-hAFP mice (H/H) but not in wild-type mice.

Strain specific AFP expression analysis in homozygous B-hAFP mice by western blot. Fetal liver tissue were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hAFP mice (H/H), and analyzed by western blot with anti-hAFP antibody. Mouse AFP was detectable in wild-type mice. Human AFP was only detectable in homozygous B-hAFP mice but not in wild-type mice.

Summary

mRNA and protein expression analysis:

Human AFP mRNA was detectable in B-hAFP mice, and we proved that the protein could be expressed normally by western blot analysis.

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