Basic Information
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BAFF protein expression analysis
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Species-specific BAFF protein expression analysis in wild-type and B-hBAFF/hBAFFR mice. Serum was collected from wild-type C57BL/6 (+/+) and homozygous B-hBAFF/hBAFFR (H/H) mice, and analyzed by ELISA using species-specific BAFF ELISA kits. Mouse BAFF protein was detected in wild-type mice, while human BAFF protein was detected in B-hBAFF/hBAFFR mice. Values are expressed as mean ± SEM. ND: not detectable.
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BAFFR protein expression analysis
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Species-specific BAFFR protein expression analysis in wild-type and B-hBAFF/hBAFFR mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hBAFF/hBAFFR (H/H) mice, and analyzed by flow cytometry using species-specific anti-BAFFR antibodies. Mouse BAFFR protein was detected in wild-type mice, while human BAFFR protein was detected in B-hBAFF/hBAFFR mice.
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Analysis of spleen leukocytes
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Analysis of spleen leukocyte subpopulations. Splenocytes were isolated from female C57BL/6 and B-hBAFF/hBAFFR mice (n=3, 7 week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. (A) Representative flow cytometry plots. Single live cells were gated on CD45+ cells and used for further analysis as indicated. (B) Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hBAFF/hBAFFR mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hBAFF and hBAFFR in place of its mouse counterpart does not change the overall development, differentiation or distribution of these spleen cell types. Values are expressed as mean ± SEM.
