Basic Information
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mRNA expression analysis
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Species-specific C1QA, C1QB and C1QC gene expression analysis in wild-type and humanized B-hC1Q mice by RT-PCR. Murine C1qa, C1qb and C1qc mRNA transcripts were detected in thymocytes isolated from wild-type C57BL/6 (+/+) mice, while human C1QA, C1QB and C1QC mRNA transcripts were detected in homozygous B-hC1Q (H/H) mice.
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Protein expression analysis
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Strain specific C1Q expression analysis in wild-type C57BL/6 mice and homozygous B-hC1Q mice by ELISA. Serum was collected from wild-type C57BL/6 mice (+/+) (n=4，8-week-old) and homozygous B-hC1Q mice (H/H) (n=4，8-week-old). Mouse C1Q was only detectable in wild-type mice. C1Q was both detectable in wild-type C57BL/6 mice and homozygous B-hC1Q mice. Therefore, it is speculated that this anti-human C1Q antibody is cross-reactive between human and mouse. Values are expressed as mean ± SEM. Significance was determined by unpaired t-test. ns: non-significant, *p < 0.05, **p< 0.01, ***p < 0.0001.
