Basic Information

Strain name
C57BL/6N-C3tm2(C3)/Bcgen
Common Name
B-hC3 mice
Background
C57BL/6N
Catalog Number
112467
Aliases
ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hC3 mice. The exons 1-41 of human C3 gene that encodes the full-length protein was inserted following part of the exon 1 of the mouse C3 in B-hC3 mice.

Protein expression analysis in serum

Strain specific C3 expression analysis in homozygous B-hC3 mice by ELISA. Serum were collected from wild-type mice (+/+) and homozygous B-hC3 mice (H/H), and analyzed by ELISA with species-specific anti-C3 antibody. Mouse C3 was detectable in wild-type mice. Human C3 was detectable in homozygous B-hC3 mice.

Summary

Protein expression analysis:

Human C3 was exclusively detectable in homozygous B-hC3 mice but not wild-type mice, and mouse C3 was detectable in wild-type mice.