Basic Information
Strain name
C57BL/6N-C3tm2(C3)/Bcgen
Common Name
B-hC3 mice
Background
C57BL/6N
Catalog Number
112467
Aliases
ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p
NCBI Gene ID
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Targeting strategy
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Gene targeting strategy for B-hC3 mice. The exons 1-41 of human C3 gene that encodes the full-length protein was inserted following part of the exon 1 of the mouse C3 in B-hC3 mice.
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Protein expression analysis in serum
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Strain specific C3 expression analysis in homozygous B-hC3 mice by ELISA. Serum were collected from wild-type mice (+/+) and homozygous B-hC3 mice (H/H), and analyzed by ELISA with species-specific anti-C3 antibody. Mouse C3 was detectable in wild-type mice. Human C3 was detectable in homozygous B-hC3 mice.
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Summary
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Protein expression analysis:
Human C3 was exclusively detectable in homozygous B-hC3 mice but not wild-type mice, and mouse C3 was detectable in wild-type mice.
