Basic Information
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Targeting strategy
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Gene targeting strategy for B-hC3 mice. The exons 1-41 of human C3 gene that encodes the full-length protein was inserted following part of the exon 1 of the mouse C3 in B-hC3 mice.
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Protein expression analysis in serum
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Strain specific C3 expression analysis in homozygous B-hC3 mice by ELISA. Serum were collected from wild-type mice (+/+) and homozygous B-hC3 mice (H/H), and analyzed by ELISA with species-specific anti-C3 antibody. Mouse C3 was detectable in wild-type mice. Human C3 was detectable in homozygous B-hC3 mice.
Strain specific C3 expression analysis in homozygous B-hC3 mice by ELISA. Serum were collected from different weeks including 10, 12 and 14-week of homozygous B-hC3 mice (H/H), and analyzed by ELISA with species-specific anti-C3 antibody. Human C3 was detectable in homozygous B-hC3 mice. The expression of human C3 did not change significantly with the increase of age. (*p<0.05,***p<0.001, ****p<0.0001)
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Analysis of blood biochemistry in B-hC3 mice
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Analysis of blood biochemical in hC3 and WT. Serum were collected from wild-type mice (+/+) and homozygous B-hC3 mice (H/H), and analyzed for biochemistry. Biochemistry analysis revealed that the B-hC3 mice presented with significant elevation of BUN and sCysC. The B-hC3 mice also showed significant elevation of liver injury markers, such as alanine aminotransferase(ALT), aspartate aminotransferase(AST), and alkaline Phosphatase(ALP).(*p<0.05,***p<0.001, ****p<0.0001)
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Summary
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- Protein expression analysis:
Human C3 was exclusively detectable in homozygous B-hC3 mice but not wild-type mice, and mouse C3 was detectable in wild-type mice.
- Analysis of blood biochemistry：
Biochemistry analysis revealed that the B-hC3 mice presented with significant elevation of BUN and sCysC. The B-hC3 mice also showed significant elevation of liver injury markers, such as ALT, AST, and ALP.
