Basic Information

Strain Name
C57BL/6-Ccl2tm1(CCL2)/Bcgen
Common Name
B-hCCL2 mice
Background
C57BL/6
Catalog number
111102
Related Genes
HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hCCL2 mice. The full coding region sequence of mouse Ccl2 was replacement by counterpart human CCL2 sequences in B-hCCL2 mice, so the mouse Ccl2 gene was depleted.

mRNA expression analysis

Strain specific analysis of CCL2 gene expression in wild-type mice and B-hCCL2 mice by RT-PCR. Mouse Ccl2 mRNA was detectable in stomach of wild-type mice (+/+). Human CCL2 mRNA was detectable only in homozygous B-hCCL2 mice (H/H) but not in wild-type C57BL/6 mice (+/+).

Protein expression analysis

Strain specific CCL2 expression analysis in homozygous B-hCCL2 mice by ELISA. Serum was collected from wild-type mice (+/+) and homozygous B-hCCL2 mice (H/H) stimulated with anti-mCD3ε antibody for 4 hours in vivo, and analyzed by ELISA with species-specific CCL2 ELISA kit. Mouse CCL2 were only detectable in wild-type mice. Human CCL2 was exclusively detectable in homozygous B-hCCL2 mice but not in wild-type mice.