Basic Information

Strain Name
C57BL/6-Ccl22tm1(CCL22)/Bcgen
Common Name
B-hCCL22 mice
Background
C57BL/6
Catalog number
110817
Related Genes
CCL22, A-152E5.1, ABCD-1, DC/B-CK, MDC, SCYA22, STCP-1, C-C motif chemokine ligand 22
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hCCL22 mice. 

The exons 1-3 of mouse Ccl22 gene that encode the full-length protein were replaced by human CCL22 exons 1-3 in B-hCCL22 mice.

mRNA expression analysis

Strain specific analysis of CCL22 gene expression in wild type (WT) mice and B-hCCL22 mice by RT-PCR. 

Mouse Ccl22 mRNA was detectable only in thymus of WT mice (+/+). Human CCL22 mRNA was detectable only in homozygous B-hCCL22 mice (H/H) but not in WT mice (+/+).

Protein expression analysis

Strain specific CCL22 expression analysis in wild type (WT) mice and B-hCCL22 mice by ELISA. 

Thymus homogenates were collected from WT mice (+/+) and homozygous B-hCCL22 mice (H/H), and analyzed by ELISA with species-specific CCL22 ELISA kit. Mouse CCL22 was detectable in WT mice. Human CCL22 was exclusively detectable in homozygous B-hCCL22 mice (H/H) but not WT mice (+/+) . Values are expressed as mean ± SEM. ND: not detectable.

Summary

  • mRNA expression analysis:

Mouse Ccl22 mRNA was detectable only in thymus of WT mice (+/+). Human CCL22 mRNA was detectable only in homozygous B-hCCL22 mice (H/H) but not in WT mice (+/+). 

  • Protein expression analysis:

Human CCL22 was exclusively detectable in thymus homogenates of homozygous B-hCCL22 mice (H/H) but not in WT mice (+/+), and mouse CCL22 was detectable only in WT mice (+/+).

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