Basic Information
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Targeting strategy
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Gene targeting strategy for B-hCCL3 mice.
The exons 1-3 of mouse Ccl3 gene that encode the full-length protein were replaced by human CCL3 exons 1-3 in B-hCCL3 mice.
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mRNA expression analysis
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Strain specific analysis of CCL3 gene expression in wild-type mice and B-hCCL3 mice by RT-PCR. Mouse Ccl3 mRNA was detectable in splenocytes of wild-type mice (+/+). Human CCL3 mRNA was detectable only in homozygous B-hCCL3 mice (H/H) but not in wild-type mice.
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Protein expression analysis
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Strain specific CCL3 expression analysis in homozygous B-hCCL3 mice by ELISA. Bone marrow-derived macrophages(BMDM) were isolated from wild-type mice (+/+) and homozygous B-hCCL3 mice (H/H) and stimulated with 10ng/ml LPS in vitro, cell culture supernates were analyzed by ELISA with species-specific CCL3 ELISA kit. Mouse CCL3 was detectable in wild-type mice. Human CCL3 was exclusively detectable in homozygous B-hCCL3 mice but not in wild-type mice.
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Summary
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- mRNA expression analysis:
Mouse Ccl3 mRNA was detectable in splenocytes of wild-type (+/+). Human CCL3 mRNA was detectable in B-hCCL3 mice (H/H) but not in wild-type mice.
- Protein expression analysis:
Mouse CCL3 was detectable in BMDM cell culture supernates of wild-type mice. Human CCL3 was exclusively detectable in homozygous B-hCCL3 mice but not in wild-type mice.
