Basic Information
Strain Name
C57BL/6-Ccl3tm1(CCL3)/Bcgen
Common Name
B-hCCL3 mice
Background
C57BL/6N
Catalog number
111439
Related Genes
CCL3 (G0S19-1, LD78ALPHA, MIP-1-alpha, MIP1A, SCYA3)
NCBI Gene ID
-
mRNA expression analysis
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Species-specific CCL3 gene expression analysis in wild-type and humanized B-hCCL3 mice by RT-PCR. Murine Ccl3 mRNA was detected in splenocytes isolated from wild-type (+/+) mice, while human CCL3 mRNA was exclusively detected in homozygous B-hCCL3 (H/H) mice.
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Protein expression analysis
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Species-specific CCL3 protein expression analysis in humanized B-hCCL3 mice. Bone marrow-derived macrophages (BMDM) were isolated from wild-type (+/+) and homozygous B-hCCL3 (H/H) mice, stimulated with 10ng/ml LPS in vitro, and analyzed by ELISA using species-specific CCL3 kits. Murine CCL3 protein was detected in wild-type mice, while human CCL3 protein was exclusively detected in B-hCCL3 mice.
