Basic Information
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Description
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- CCL8 (C-C motif chemokine ligand 8), also known as MCP-2, is a chemotactic cytokine, which was mainly produced in monocytes/macrophages, fibroblasts, endometrial cells and mast cells. CCL8 can exert a chemotactic effect on monocytes, T lymphocytes, NK cells, basophils and eosinophils.
- Biocytogen developed a CCL8 humanized mice, the exons 1~3 of mouse Ccl12 gene that encode the full length protein were replaced by human CCL8 exons 1~3 in B-hCCL8 mice. Then, confirmed that human CCL8 protein was exclusively detectable in serum of homozygous B-hCCL8 mice.
- The mice can be used for preclinical pharmacodynamics studies of target-related disease.
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Targeting strategy
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Gene targeting strategy for B-hCCL8 mice.
The exons 1-3 of mouse Ccl12 gene that encode the whole molecule (ATG to STOP codon) were replaced by human counterparts in B-hCCL8 mice. The promoter and 5’UTR region of the mouse gene are retained. The human CCL8 expression is driven by endogenous mouse Ccl12 promoter, while mouse Ccl12 gene transcription and translation will be disrupted.
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Protein expression analysis
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Strain specific CCL8 and CCL12 expression analysis in wild-type C57BL/6N mice and homozygous humanized B-hCCL8 mice by ELISA. Serum was collected from wild-type mice (+/+) and homozygous B-hCCL8 mice (H/H) stimulated with 40 μg LPS in vivo for 2 hours (male, 25-week old, n=3). Expression level of hCCL8 and mCCL12 were analyzed by ELISA (anti-mouse CCL12 ELISA kit: Abcam, ab246547; anti-human CCL8 ELISA kit: Abcam, ab223856). Mouse CCL12 was only detectable in wild-type C57BL/6N mice. Human CCL8 was exclusively detectable in homozygous B-hCCL8 mice but not in wild-type mice. Values are expressed as mean ± SEM.
