Basic Information
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Targeting strategy
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Gene targeting strategy for B-hCCN1 mice.
The exons 1-5 of mouse Ccn1 gene were replaced by human CCN1 exons 1-5 in B-hCCN1 mice.
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mRNA expression analysis
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Strain specific analysis of CCN1 gene expression in wild-type mice and B-hCCN1 mice by RT-PCR.
Mouse Ccn1 mRNA was detectable in lung of wild-type mice (+/+). Human CCN1 mRNA was detectable only in homozygous B-hCCN1 mice but not in wild-type mice.
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Protein expression analysis
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Strain specific CCN1 expression analysis in homozygous B-hCCN1 mice by western blot.
Spleen tissue was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCCN1 mice (H/H), and analyzed by western blot with anti-CCN1 antibody. Mouse CCN1 was detectable in wild type mice and homozygous B-hCCN1 mice, as the antibody is crossly reactive with CCN1 in human and mice.
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Summary
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mRNA expression：
Mouse Ccn1 mRNA was detectable in lung of wild-type mice (+/+). Human CCN1 mRNA was detectable only in homozygous B-hCCN1 mice but not in wild-type mice.
Protein expression：
Mouse CCN1 was detectable in wild type mice and homozygous B-hCCN1 mice, as the antibody is crossly reactive with CCN1 in human and mice.
