Basic Information

Strain Name
C57BL/6-Ccn1tm1(CCN1)/Bcgen
Common Name
B-hCCN1 mice
Background
C57BL/6
Catalog Number
111235
Related Gene
CYR61, GIG1, IGFBP10
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hCCN1 mice.

The exons 1-5 of mouse Ccn1 gene were replaced by human CCN1 exons 1-5 in B-hCCN1 mice.

mRNA expression analysis

Strain specific analysis of CCN1 gene expression in wild-type mice and B-hCCN1 mice by RT-PCR.

Mouse Ccn1 mRNA was detectable in lung of wild-type mice (+/+). Human CCN1 mRNA was detectable only in homozygous B-hCCN1 mice but not in wild-type mice.

Protein expression analysis

Strain specific CCN1 expression analysis in homozygous B-hCCN1 mice by western blot.

Spleen tissue was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCCN1 mice (H/H), and analyzed by western blot with anti-CCN1 antibody. Mouse CCN1 was detectable in wild type mice and homozygous B-hCCN1 mice, as the antibody is crossly reactive with CCN1 in human and mice.

Summary

mRNA expression

Mouse Ccn1 mRNA was detectable in lung of wild-type mice (+/+). Human CCN1 mRNA was detectable only in homozygous B-hCCN1 mice but not in wild-type mice.

Protein expression

Mouse CCN1 was detectable in wild type mice and homozygous B-hCCN1 mice, as the antibody is crossly reactive with CCN1 in human and mice.