Basic Information

Strain Name
C57BL/6-Ccn2tm2(CCN2)/Bcgen
Common Name
B-hCCN2 mice
Background
C57BL/6
Catalog Number
111523
Related Gene
CCN2,cellular communication network factor 2,CTGF, HCS24, IGFBP8, NOV2
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hCCN2 mice.

The exons 1-5 of mouse Ccn2 gene were replaced by human CCN2 exons 1-5 in B-hCCN2 mice.

mRNA expression analysis

Strain specific analysis of CCN2 gene expression in wild-type mice and B-hCCN2 mice by RT-PCR.

Mouse Ccn2 mRNA was detectable in kidney of wild-type mice (+/+). Human CCN2 mRNA was detectable only in homozygous B-hCCN1 mice but not in wild-type mice.

Protein expression analysis

Strain specific CCN2 expression analysis in homozygous B-hCCN2 mice by Western blot.

Kidney tissue was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCCN2 mice (H/H), and analyzed by western blot with anti-CCN2 antibody. CCN2 was detectable in wild type mice and homozygous B-hCCN2 mice, as the antibody is crossly reactive with CCN2 in human and mice.

Summary

mRNA expression

Mouse Ccn2 mRNA was detectable in kidney of wild-type mice (+/+). Human CCN2 mRNA was detectable only in homozygous B-hCCN1 mice but not in wild-type mice.

Protein expression

CCN2 was detectable in wild type mice and homozygous B-hCCN2 mice, as the antibody is crossly reactive with CCN2 in human and mice.