Basic Information

Strain Name
C57BL/6-Ccn2tm2(CCN2)/Bcgen
Common Name
B-hCCN2 mice
Background
C57BL/6
Catalog Number
111523
Related Gene
CCN2,cellular communication network factor 2,CTGF, HCS24, IGFBP8, NOV2
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hCCN2 mice.

The exons 1-5 of mouse Ccn2 gene were replaced by human CCN2 exons 1-5 in B-hCCN2 mice.

mRNA expression analysis

Species-specific CCN2 gene expression analysis in wild-type and B-hCCN2 mice by RT-PCR. Murine Ccn2 mRNA was detected in kidney cells isolated from wild-type (+/+) mice, while human CCN2 mRNA was exclusively detected in homozygous B-hCCN2 (H/H) mice.

Protein expression analysis

Species-specific CCN2 protein expression analysis in B-hCCN2 mice by Western blot. Kidney tissue was isolated from wild-type C57BL/6 (+/+) and homozygous B-hCCN2 (H/H) mice, and analyzed by western blot using a cross-reactive anti-m/hCCN2 antibody. Mouse and human CCN2 protein was detected in wild-type and homozygous B-hCCN2 mice.

Summary

mRNA expression

Murine Ccn2 mRNA was detected in kidney cells isolated from wild-type (+/+) mice, while human CCN2 mRNA was exclusively detected in homozygous B-hCCN2 (H/H) mice.

Protein expression

Mouse and human CCN2 protein was detected in wild-type and homozygous B-hCCN2 mice.