Basic Information

Strain Name
C57BL/6-Ccn4tm1(CCN4)/Bcgen
Common Name
B-hCCN4 mice
Background
C57BL/6
Catalog number
111237
Related Gene
WISP1, WISP1-OT1, WISP1-UT1, WISP1c, WISP1i, WISP1tc
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hCCN4 mice.

The exons 1-5 of mouse Ccn4 gene were replaced by human CCN4 exons 1-5 in B-hCCN4 mice.

mRNA expression analysis

Strain specific analysis of CCN4 gene expression in wild-type mice and B-hCCN4 mice by RT-PCR.

Mouse Ccn4 mRNA was detectable in ovary of wild-type mice (+/+). Human CCN4 mRNA was detectable only in homozygous B-hCCN4 mice but not in wild-type mice.

Protein expression analysis

Strain specific CCN4 expression analysis in homozygous B-hCCN4 mice by Western blot.

Kidney and spleen tissue were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCCN4 mice (H/H), and analyzed by western blot with anti-CCN4 antibody. CCN4 was detectable in wild type mice and homozygous B-hCCN4 mice, as the antibody is crossly reactive with CCN4 in human and mice.

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