Basic Information

Strain Name
C57BL/6-Ccn4tm1(CCN4)/Bcgen
Common Name
B-hCCN4 mice
Background
C57BL/6J
Catalog number
111237
Related Genes
WISP1, WISP1-OT1, WISP1-UT1, WISP1c, WISP1i, WISP1tc
NCBI Gene ID

mRNA expression analysis

Species-specific CCN4 gene expression analysis in wild-type and B-hCCN4 mice by RT-PCR. Murine Ccn4 mRNA was detected in ovary isolated from wild-type (+/+) mice, while human CCN4 mRNA was exclusively detected in homozygous B-hCCN4 mice.

Protein expression analysis

Species-specific CCN4 protein expression analysis in homozygous B-hCCN4 mice by Western blot. Kidney and spleen tissue were isolated from wild-type C57BL/6 (+/+) and homozygous B-hCCN4 (H/H) mice, and analyzed by western blot using anti-CCN4 antibodies. CCN4 protein was detected in wild type and homozygous B-hCCN4 mice, as the antibody is cross-reactive with CCN4 in human and mice.