Basic Information
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Targeting strategy
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Gene targeting strategy for B-hCCN4 mice.The exons 1-5 of mouse Ccn4 gene were replaced by human CCN4 exons 1-5 in B-hCCN4 mice.
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mRNA expression analysis
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Strain specific analysis of CCN4 gene expression in wild-type mice and B-hCCN4 mice by RT-PCR. Mouse Ccn4 mRNA was detectable in ovary of wild-type mice (+/+). Human CCN4 mRNA was detectable only in homozygous B-hCCN4 mice but not in wild-type mice.
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Protein expression analysis
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Strain specific CCN4 expression analysis in homozygous B-hCCN4 mice by Western blot. Kidney and spleen tissue were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCCN4 mice (H/H), and analyzed by western blot with anti-CCN4 antibody. CCN4 was detectable in wild type mice and homozygous B-hCCN4 mice, as the antibody is crossly reactive with CCN4 in human and mice.
