Basic Information
Strain Name
C57BL/6-Cdh17tm1(CDH17)/Bcgen
Common Name
B-hCDH17 mice
Background
C57BL/6
Catalog Number
111593
Alias
HPT1; CDH16; HPT-1
NCBI Gene ID
-
Gene targeting strategy
-
Gene targeting strategy for B-hCDH17 mice. The extracellular region of mouse Cdh17 was replaced by human CDH17 extracellular region sequences in B-hCDH17 mice.
-
mRNA expression analysis
-
Species-specific CDH17 gene expression analysis in wild-type and humanized B-hCDH17 mice by RT-PCR. Mouse Cdh17 mRNA was detected in colon tissue isolated from wild-type C57BL/6 (+/+) mice, while human CDH17 mRNA was detected in homozygous B-hCDH17 (H/H) mice.
-
Protein expression analysis
-
CDH17 protein expression analysis in humanized B-hCDH17 mice. Mouse small intestine and colon lysates were analyzed by Simple Western using an anti-human CDH17 antibody. Human CDH17 protein was detected in homozygous B-hCDH17 (H/H) mice, but not in wild-type C57BL/6 (+/+) mice.
