Basic Information

Strain Name
C57BL/6-Cdh17tm1(CDH17)/Bcgen
Common Name
B-hCDH17 mice
Background
C57BL/6
Catalog Number
111593
Alias
HPT1; CDH16; HPT-1
NCBI Gene ID

Gene targeting strategy

Gene targeting strategy for B-hCDH17 mice. The extracellular region of mouse Cdh17 was replaced by human CDH17 extracellular region sequences in B-hCDH17 mice.

mRNA expression analysis

Species-specific CDH17 gene expression analysis in wild-type and humanized B-hCDH17 mice by RT-PCR. Mouse Cdh17 mRNA was detected in colon tissue isolated from wild-type  C57BL/6 (+/+) mice, while human CDH17 mRNA was detected in homozygous B-hCDH17 (H/H) mice.

Protein expression analysis

CDH17 protein expression analysis in humanized B-hCDH17 mice. Mouse small intestine and colon lysates were analyzed by Simple Western using an anti-human CDH17 antibody. Human CDH17 protein was detected in homozygous B-hCDH17 (H/H) mice, but not in wild-type C57BL/6 (+/+) mice.

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